When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
answer: Neuro filaments
explanation:
Neurofilaments (NF) are intermediate filaments found in the cytoplasm of neurons. Together with microtubules and microfilaments, they form the neuronalcytoskeleton. They are believed to function primarily to provide structural support for axons and to regulate axon diameter, which influences nerve conduction velocity.
Answer:
The crust is thinner and less dense than the mantle.
The answer is confirmation bias. This is the tendency to look for evidence that will confirm ones hypothesis and ignoring anything else that does not lead to the hypothesis. In this situation confirmation bias is evident due to the fact that you look for anything that confirms your hypothesis and ignore anything else that does not point to drug abuse.
