Question

high efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier

Answer 1

The efficiency of genetic transformation of intact Saccharomyces cerevisiae cells rose to more than 1 105 transformants per microgram of vector DNA and to 1.5% transformants per viable cell utilising a method that produces competent cells using LiAc.

What is nucleic acids ?

Biopolymers and macromolecules called nucleic acids are necessary for all known forms of life. They are constructed of monomers called nucleotides, which are composed of a nitrogenous base, a phosphate group, and a 5-carbon sugar. Deoxyribonucleic acid and ribonucleic acid are the two primary types of nucleic acids.

• Gene expression, cellular activity regulation, genetic information storage and transfer are the three primary uses of nucleic acids.

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