Question

Identification and Characterization of the Trex-Binding Factor in the Muscle Creatine Kinase Enhancer.

Answer 1

The Trex-Binding Factor in Muscle Creatine Kinase Enhancer has been identified and described below.

                                                                                                                         The Muscle Creatine Kinase Enhancer contains a positive control site called Transcriptional Regulatory Element X (Trex). The Trex site is critical for MCK expression in skeletal and cardiac muscle, according to research using transgenic animals and cell culture. Quantitative proteomics was used to identify the Trex-binding factor (TrexBF) as Six$4,$ a homeodomain transcription factor of the Six/sine oculi's family, from a background of about$900$ copurifying proteins, following selective enrichment for TrexBF using magnetic beads coupled to oligonucleotides containing either wild-type or mutant Trex sites. We showed that Six4 is TrexBF in mouse skeletal myocytes and embryonic day 10 chick skeletal and cardiac muscle, but Six$5$is the primary TrexBF in adult mouse heart, using gel shift assays and Six-specific antisera. Six$4$transactivates in co transfection investigations.

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