Question

Improved Post-Resuscitation Survival Time with Adjuvant Cytosolic Energy Replenishment in a Murine Model of

Answer 1

A murine model of hemorrhagic refractory shock showed improved post-resuscitation survival time with adjuvant cytosolic energy replenishment.

Objective:

• Resuscitation of a patient with refractory hemorrhagic hypovolemic shock (HS) is difficult.
• HS is linked to severe reductions of cellular energy nucleotides, which can result in cardio-circulatory arrest and mortality.
• Vasopressors, most often norepinephrine, are typically briefly supplied to control a persistent hypotension that is not rectified by intensive resuscitation attempts in order to avert an impending cardio-circulatory arrest.

Method

The following five groups of 10 each were randomly assigned to 50 male Sprague-Dawley rats:

• HS/conventional resuscitation (CR), HS/CR+norepinephrine, HS/CR+vasopressin, HS/CR+vesicles, and HS/CR+ATPv.
• ATPv, which are highly fusogenic lipid vesicles encasing ATP, were used to directly replace the cytosol with ATP.
• Direct cytosolic ATP delivery is made possible by the instantaneous fusion of the ATPv with the cell membrane.
• The study's endpoint was established as the post-resuscitation survival period.

Results:

The SI and MAP showed that all animals experienced the same kind of shock. According to the long-rank Mantel-Cox test and Kaplan-Meier survival curves, the median post resuscitation survival periods were as follows: HS/CR+Nor epinephrine = 35.5 min; HS/CR+Vasopressin = 20 min; HS/CR+Lipid Vesicles control = 88.5 min; and HS/CR+ATPv = 158.5 min

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