Answer:
Polymerase chain reaction (PCR) test is used to duplicate traces of DNA from any living form, for identification purposes. The correct sequence is 1, 3, 2; based on the options given.
Explanation:
The steps to be followed for the PCR procedure are:
<em>Denaturation</em>
Option 1. Involves incubation at 94°C to denature DNA strands into single strands by breaking of weak hydrogen links
<em>Annealing</em>
Option 3. Temperature is lowered to 60°C to allow primers joining their similar DNA sequences.
<em>Elongation</em>
Option 2. Incubate at 72°C to promote the extension of the DNA strands, actually extending them.
City-state government was often conquered because they did not have enough resources to protect themselves
Explanation:
A city-state government is an independent autonomic political system which usually compromises just a single independent city which is governed by itself with sovereignty over its territory. The city-states like Greece, Italy, and Rome etc became very powerful.
The main reason for these city-states being conquered more was the accumulation of wealth and aristocracy in such states.
Heavy taxes, religion, slavery also lead to social unrest and conflicts among these city-states which made them weaker. These internal conflicts also made them lose their resources and vulnerable to be conquered by other powerful empires.
With the increasing power of neighboring states, like in the case of Rome and Greece, the Romans became mightier than Greeks at one point and conquered the Greek city-states.
The answer is:
Echinodermata
The word echinodermata is Greek which literally means "Spiny Skin". Echinodermata is a phylum under kingdom animalia. Not all of them have spiny skins, but most of them do. Other characteristics would be their radially symmetric body. They have appendages that grow outward from the center. Examples of echinoderms would be sea stars and sea urchins.
The inflammation will not occur with tissue that is dead & has no blood supply. The injury brings a decrease in white blood cells. The Mast Cells play a role in releasing histamine which dilate capillaries and bring about hypermia cause increase blood and swelling and redness to the area
A type of strong chemical bond in which two atoms share one or more pairs of valence electrons. Two or more atoms held together by covalent bonds. The attraction that exists between opposing (positive and negative) charges within the atom.
