How do researchers prepare an intron-free copy of a eukaryotic gene for use in creating transgenic bacteria?

1 answer:

3 0

The researchers prepare an intron-free copy of the eukaryotic gene for the use in creating the transgenic bacteria by using the reverse transcriptase to make the cDNA from the mature mRNA

The structure of the eukaryotic genes. Most of the eukaryotic genes are contain the segments of the coding sequences (exons) that interrupted by the noncoding sequences (introns). Both the exons and the introns are transcribed to yield a long and primary RNA transcript.

Eukaryotic DNA is the linear, compacted into the chromosomes by the histones, and has the telomeres at each end to protect from the deterioration. The Prokaryotes contain the circular DNA in addition to the smaller, transferable DNA plasmids. Eukaryotic cells contain the mitochondrial DNA in the addition to the nuclear DNA

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