Observe how deeply in the agar your organism grew. Is there growth on the surface? Where is the majority of the growth located?
Remember that your unknown organism was inoculated all the way to the bottom of the tube initially, so there may be bacteria visible along the whole inoculation line. But evaluate where you see the most growth.
The organism grew deep in the agar solution near to the bottom of the petri dish. No, the growth occurs at the bottom of the agar solution because the bacteria does not need oxygen and can survive in anaerobic condition. Majority of the growth located at the sides of the petri dish in the form of colonies. The most growth of bacteria occurs at the bottom of the medium.
I just answered this question from a different person but I like helping with science. This is from the water's breeze or air currents. This makes the climate mild while the inland is more extreme because it does not have the breeze. Hopefully this helps :D
