When neurons are loaded with fura-2, an increase in cytoplasmic fluorescence is expected when the neuron is depolarized. Fura-2 is a calcium indicator dye that is often used to measure changes in intracellular calcium concentration. When neurons are depolarized, there is an influx of calcium into the cell, which results in an increase in fluorescence.
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Explain Cytoplasmic fluorescence?</h3>
Cytoplasmic fluorescence is a type of fluorescence that occurs in the cytoplasm of cells. This fluorescence is caused by the presence of fluorescent molecules in the cytoplasm, which absorb and emit light at specific wavelengths. The most common fluorescent molecule in the cytoplasm is green fluorescent protein (GFP), which emits green light when excited by ultraviolet (UV) light. Cytoplasmic fluorescence is used in many biological applications, including cell imaging, protein localization, and gene expression analysis.
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