When calibrating a spectrophotometer, measuring absorbance concurrently is the best option for a blank since it is proportional to the concentration.
Beer's law states that A = a b c, wherein there is the diffusion coefficient at a constant, b is the actual route length, & c is the concentration. Direct proportionality exists between b and c and absorbance.
Once the route length is doubled, incident light contacts double as many molecules in the solution. The consequence is a doubling of absorbance, which is equivalent to a doubling of molecule concentration.
There are two ways to detect chemicals using spectrum scanning. One approach involves turning the monochromator continuously with a stepping motor while gradually altering the wavelength connected to the output slit.
It is more practical to use diode array detectors. Up to a few hundred photodiodes may be incorporated into the chip that makes up this device.
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