Question

Why are thick or dense smears less likely to provide a good smear preparation for microscopic.

Answer 1

Thick or dense smears less likely to provide a good smear preparation for microscopic because it will diminish the amount of light that can pass through making it difficult to visualize the morphology of single cells under the microscope. Some times the stain can't penetrate all of the bacteria.

What is a microscopic smear?

• A smear is a microscopic specimen.
• A swab or loop, or the edge of another slide, is used to spread the sample to be examined, such as blood or microbial culture, thinly and unevenly across the slide.
• Smear preparation involves spreading a small amount of sample on a slide and air drying the film before staining and microscopy.
• The conventional method, blood film method, drop and rest method, and water-wash method are the four types of smearing methods.
• Smear microscopy entails collecting a biological sample (typically sputum or other clinical material), fixing it thinly on a glass slide, and staining it with a dye that binds specifically to mycobacteria (making them easier to identify under a microscope).

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