Question

when foreign dna and plasmid are both cut with the same restriction enzyme and then mixed together, all molecules will form recombinants.

Answer 1

False, Some plasmids may reanneal without taking in any more DNA. when plasmid and foreign DNA are combined after being cut with the same restriction enzyme

Enzymes that cleave DNA are known as restriction enzymes. Each enzyme distinguishes one or more target sequences and cleaves DNA at or close to those sequences.

Numerous restriction enzymes produce single-stranded DNA overhangs at the ends of their cuts, which are often staggered. But some result in blunt endings.

DNA ligase is an enzyme that joins DNA. A single, unbroken DNA molecule can be created by joining two sections of DNA that have matching ends using ligase.

DNA ligase and restriction enzymes are employed in DNA cloning to splice genes and other DNA fragments into plasmids.

An enzyme that cuts DNA and recognises particular DNA locations is known as a restriction enzyme. A number of restriction enzymes make staggered cuts at or near their recognition sites to create ends with a single-stranded overhang.

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