Increased mitochondrial membrane permeability results in a buildup of oxygen inside the mitochondrial matrix and a decrease in NADH oxidation as well as a decrease in the ability to pump hydrogens into the intermembrane gap ( this will stay in its reduced form).
What is mitochondrial membrane ?
As is typical of energy-transducing membranes, the mitochondrial membrane is a lamellar array rich in proteins. By fractionating the lamellae and isolating the numerous protein complexes buried within, the chemistry of its operation as a respiratory unit was ascertained. So that four electron-transport protein complexes may be resolved, the inner mitochondrial membrane can be divided and fractionated.
Initially, EMR was used to analyze these preparations in order to spectroscopically determine the metal ion composition of each of the protein complexes, which each include transition metal ions. The addition of exogenous substances like reductants and recognized enzyme substrates could change the EMR signal, it was soon discovered. Investigations of soluble proteins that were similar to those of the studies of mitochondrial protein complexes,63–73,98 were also conducted.
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