Question

The primers you used in lab amplified 145 base pairs that were adjacent, but not included in the repeat. If you used a different primer that only amplified 130 base pairs adjacent to a 14 base pair repeat, and your pcr product size was 172, how many repeats were in your product?.

Answer 1

If your PCR product size was 172 and there were 130 base pairs next to a 14 base pair repeat, then there are 3 repeats.

Given that the newly used primer amplifies 130 base pairs that are near

(a) 14 base pair and

(b) the size of the pcr product is 172

Size = (number of base pairs * number of repetitions) + Initial/Primer Amplicon Value

172 = (14 * x) + 130

172 -130 = 14x

42 = 14x

3 = x

Therefore, we can say that the PCR product has 3 repeats.

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