RNA and DNA-RNA hybrids are more dense than simply DNA if the density of different nucleic acids is tested using a CsCl gradient.
The primary technique employed in molecular biology is the extraction of DNA, RNA, and protein. Scientists can separate materials according to size, shape, and density by using density gradient centrifugation. Isopycnic centrifugation, a sort of density gradient centrifugation that Meselson and Stahl developed, employed a cesium chloride solution to separate DNA molecules solely on the basis of density. Because DNA has hydrogen bonds between its strands, which makes it less dense than RNA, RNA has a far larger density than DNA. RNA can be obtained at the test tube's bottom using a CsCl density gradient and high centrifugation, whereas DNA can be recovered in the middle layer.
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