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10 months ago

List and describe the purpose of each component of the restriction enzyme digest. Why do some tubes have no restriction enzyme?.

1 answer:

8 0

A typical restriction digestion reaction includes the DNA template, the desired restriction enzyme, a buffer, and occasionally BSA protein.

<h3>How come a restriction enzyme wouldn't function?</h3>

You should always check the expiration date, confirm that the enzyme has been stored at -20°C, and check the temperature of your freezer (do not allow temperatures to surpass -20°C, since many freeze-thaw cycles may cause an enzyme to lose action).

<h3>How does a restriction digest work?</h3>

With the aid of specialized enzymes known as restriction endonucleases (often referred to as restriction enzymes or REs), restriction digestion is the process of breaking down DNA molecules into smaller fragments.

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There are two species of freshwater shrimp that are introduced to a pond. The first species, Caridina cantonensis, is brightly c

Marina86 [1]

Explanation:

Natural selection is the mechanism that is responsible for the evolution of organisms.

In the given case, the Freshwater shrimp has been introduced to a pond in which the two species with variations are introduced.

The <em>C. cantonensis </em>is brighter in color whereas the <em>C. multidentata</em> is mottled drab in color. The predator fish can feed easily on the species which is brighter therefore <em>C. cantonensis</em> is more susceptible. The mottled drab species is not easily predated by the species.

The 30 % offsprings of <em>C. cantonensis</em> can survive till the reproductive age whereas 75% of C. multidentata. This shows that natural selection has acted on the color of the shrimp species selected against the predator fish species.

The species with mottled drab color is the result of the differential reproductive rate.

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2 years ago

The blockage of cerebrospinal fluid (csf) outflow in the brain can cause accumulation of fluid, resulting in cranial enlargement

alisha [4.7K]

The correct answer is hydrocephalus.

Hydrocephalus refers to the condition in which the accumulation of fluid takes place in the ventricles (cavities) deep inside the brain. The excess fluid enhances the size of the ventricles and applies pressure on the brain. The cerebrospinal fluid usually flows via the ventricles and bathes the spinal column and the brain.

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2 years ago

Biologists focus on heredity in human development, whereas sociologists focus on learning, socialization, and culture. This diff

Aleks04 [339]

Answers

Nature vs Nurture debate.

Explanation:

Development in human is inborn.The stages of development is dictated and directed by expression of genes encoded by the DNA.Therefore physiological and biochemical interpretations of these DNA dictates is known as human development. it is a fixed mechanisms which is fastest at childhood,and progressively slower at advanced stage.<u>Although environmental factors may influence this developmental stages, the exact mechanism of occurrence is constant and fixed.</u>

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Nuture concept believe that, human development is as a result of societal and cultural factors in the environment, and the progression of the development is due to the influences of theses factors on human perception of life. Thus Human development is acquired through life exposure and experiences.They are therefore dynamic, and not static, but fluctuates based on level of exposure in the environment.

<u>However, both concept interacts with one another to ensure an holistic human development. Generally it is the interaction of the environment with genetic that explains the concept of natural selection and evolution of human race. </u>

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2 years ago

Why is it essential that the regulatory mechanisms that activate glycogen synthesis also deactivate glycogen phosphorylase?

Marta_Voda [28]

Answer:

There are two pathways occur in the same cellular compartment, and, if both are on at the same time, a futile ATP hydrolysis cycle results. Using the same mechanism to turn them on/off or off/on is highly efficient.

- "Glycogen Phosphorylase" activity can be allosterically controlled ATP and G6P allosteric inhibitors, AMP allosteric activator as well as, controlled through covalent modification, phosphorylation and via hormones.

- Reaction Catalyzed by Glycogen Synthase:

The activity of glycogen synthase is subject to the same type of covalent modification as glycogen phosphorylase, however, the response is opposite. Glycogen Synthase is activated by G6P. It is also controlled via hormones.

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2 years ago

Nutrientes del ovocito???

Lelechka [254]

The nutrients are grams gram gram

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2 years ago