Question

List and describe the purpose of each component of the restriction enzyme digest. Why do some tubes have no restriction enzyme?.

Answer 1

A typical restriction digestion reaction includes the DNA template, the desired restriction enzyme, a buffer, and occasionally BSA protein.

How come a restriction enzyme wouldn't function?

You should always check the expiration date, confirm that the enzyme has been stored at -20°C, and check the temperature of your freezer (do not allow temperatures to surpass -20°C, since many freeze-thaw cycles may cause an enzyme to lose action).

How does a restriction digest work?

With the aid of specialized enzymes known as restriction endonucleases (often referred to as restriction enzymes or REs), restriction digestion is the process of breaking down DNA molecules into smaller fragments.

To know more about  enzyme digest visit:-

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