Evaluate at least 200 spermatozoa, tally the numbers of live and dead spermatozoa using a laboratory counter, and calculate the percentage of live spermatozoa (see Note 10). The lower reference limit for vitality assessed by eosin–nigrosin stain (membrane-intact spermatozoa) is 58% (5th percentile, 95% CI 55–63) (1).
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You are not sure whether the condition is serious.
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Virus transmission is affected by a number of factors, including environmental determinants, host behavior, host defense mechanisms, and virus infectivity.
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