Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Should be make energy but get glucose makes the most sense
Answer: Option (a) is the correct answer.
Explanation:
It is known that weight is equal to mass times acceleration due to gravity.
Mathematically, Weight = mass × acceleration due to gravity
Since, it is given that weight is 34 N and it is known value of g is 9.8 m/s.
Therefore, calculate mass of the box as follows.
Weight = mass × acceleration due to gravity
34 N = mass × 9.8 m/s
mass = 3.47 kg
Hence, we can conclude that mass of the box is 3.47 kg.
Answer:
A.
Explanation:
Predators hunt prey. (Obviously). As such, they help control the population of that particular prey-species. If the predator species is removed from the equation, 2 times are changed. Long term & short term. Long term includes lower populations due to lack of resources. Short term includes overpopulation due to lack of threat.
Answer:
The endocrine system is a chemical messaging network. Endocrine glands secrete hormones, which are carried by the circulatory system throughout the body. The primary endocrine glands are the pituitary, hypothalamus, pineal gland, thyroid, parathyroid, adrenal, pancreas, ovary, and testis. Hormones maintain homeostasis in the body.
Explanation:
Hope this helps!
