When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Answer:
c) matter in an ecosystem flows in one direction and is never reused.
Explanation:
A is true
True
Because they break down what they are made of
Answer:
The pressure of a given amount of gas is directly proportional to the temperature at a given amount of volume. When the temperature of a system goes up, the pressure also goes up, and vice versa.
Explanation:
This occurs because of the pressure temperature law. (or also known as the Gay-Lussac's law)
