Defined the border between the United States and Spanish Florida; guaranteed US navigation rights on the lower Mississippi River
Tensions increased over time between Jamestown colonists and the natives.
Although in the beginning they were trading with the Jamestown colonists for metal tools. But afterward the natives realized they did not only come to trade but also to take over the land and conquer the people. That's when the tensions began and that's what caused the indian massacre of 1622
Not really sure what you mean by context of gender. Can you give more info as to what your asking?