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algol [13]
3 years ago
15

Two "naked" (without histones or other proteins) double-stranded fragments of DNA are exactly the same length. At 89°C, fragment

A has completely denatured, which means that the two strands have separated. At that temperature, fragment B is still double-stranded. How might these fragments differ, to result in different denaturation temperatures?
Biology
1 answer:
ycow [4]3 years ago
7 0

Answer:

Fragment B has a higher C/G base pair content.

Explanation:

DNA denaturalization happens when the double helix melts and the result is <u>two single strands</u>. The separation occurs because the hydrogen bonds between the bases, break apart.

DNA denaturalization occurs faster in the regions where the interaction between the two strands is weak, <u>A/T rich regions</u>. On the other hand in <u>C/G rich regions</u>, the strand is held together more strongly hence, it takes more time for it to break apart.

DNA with a lot of A/T base pairs denatures at 158 °F  (70°C) and DNA rich with C/G base pairs denatures at over 212 °F (100°C). C≡G base pairs have three hydrogen bonds, the bond is very stable and needs a lot of energy to be dissociated. <u>The higher the C≡G base pairs content, the higher the melting point of the DNA.</u>

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