Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
(Next time please insert a picture of The graph regarding to your question)
Answer: The answer is (A)
Explanation:
QR- Liquid Only
RS- Phase Change Liquid-Gas
ST-Gas Only
This graph does not begin in the solid phase of water. It begins at 40 degrees Celsius in the liquid phase. Plateaus are phase changes. In this case, it’s a phase change from liquid to gas.
It is 4. the reproductive cycles of males and females are regulated by hormones
If Alpa wants to change 0.54 kilometers into a smaller metric unit, she will be required to multiply.
<h3>What is a Metric unit?</h3>
A Metric unit may be defined as a technique of measurement in which the basic units are the meter, the second, and the kilogram. These units are interchanged with one another by using several formulas of conversion.
The complete question is as follows:
Alpha wants to change 0.54 kilometers into a smaller metric unit. She will have to:
- multiply
- divide
- add
- subtract
Therefore, If Alpa wants to change 0.54 kilometers into a smaller metric unit, she will be required to multiply.
For example, In case, if she wants to convert 0.54 into meters, then she has to multiply it by 1000, that is, 0.54 * 1000 = 540 meters.
To learn more about Metric units, refer to the link:
brainly.com/question/1576704
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Air polution can cause things such as asthma, lung cancer, and many other things. air polution could also cause headaches, dizziness, nausea, and more.
