Answer:
When directed mutagenesis which is also called reverse genetics is used, only a single mutation per genome is introduced. In contrast, the number of mutations per genome introduced by random mutagenesis is usually difficult to control. It is likely that the strain you obtained with a mutation in gene X after random mutagenesis contained an additional mutation or perhaps multiple mutations which ultimately was/were responsible for loss of virulence in your favorite pathogen.
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The kingdom that this specific organism would belong to is fungi.
Why?
Because almost all fungi heavily depend on autotrophs for energy. Fungi can also be multicellular.
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Answer:
Metaphase ⇒ Chromosomes line up in the middle of the cell
Prophase ⇒ DNA condenses to form chromosomes
Anaphase ⇒ Each chromosome separates and moves to opposite ends of the cell
Telophase ⇒ Nuclear membranes form around each set of chromosomes
