Due to natural selection in finding a mate, t<span>he </span>color<span> of the </span>dewlap would start to lighten slightly in order also to <span>adapt to their surroundings.</span>
<span> If you use as much force on a bigger object and a smaller object the smaller object will have more acceleration, because the smaller object has less mass. </span>
<span>Basal Body Temperature (BBT) is the body temperature upon waking up
or when a person is at rest. After ovulation, a woman’s BBT rises because of increase in
progesterone level. BBT swill stay high throughout the post-ovulation phase and
until the following cycle. If there is pregnancy, BBT will stay high.</span>
Phagocytosis is the ingestion and destruction of particle matter by the amoeba
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
