1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
krok68 [10]
4 years ago
12

How do pH affect the stability of double-stranded nucleic acid?

Biology
1 answer:
zaharov [31]4 years ago
4 0
Alkaline denaturation neutralize the charge of acids but also cause hydrolysis of bases upon prolonged treatment. Strong bases will raise pH until the H+ shared between the N-base electronegative centers (N-H and O=) is stripped from the H-bond, effectively breaking them.

Organic solvents such as dimethyl sulfoxide and formamide, or high pH, could break the hydrogen bonding between DNA strands too. Low salt concentration could also denature DNA double-strands by removing ions that stabilize the negative charges on the two strands from each other.

Low pH (less than pH 1): both RNA and DNA hydrolyze (phosphodiester bonds break and the bases break off).

High pH (greater than pH 11): RNA hydrolyzes, DNA will denature but the phosphodieser backbone remains intact.

[salt] Tm is sensitive to Na+ concentration. Na+ acts to shield the negative charges of the sugar-phosophate backbone from interacting with one another. The repulsion between the negatively charged phosphate backbones is the major force destabilizing the double helix, therefore increasing Na+ concentration increases helix stability and decreasing Na+ concentration decreases helix stability.

You might be interested in
Which characteristic most likely describes a mushroom but not Thermus aquaticus?
Lunna [17]

Eukaryotic is the characteristic most likely describes a mushroom but not Thermus aquaticus.

<h3>Mushroom belongs to which domain?</h3>

Any member of the eukaryotic group of organisms, which also includes the more well-known mushrooms and microbes like yeast and mold, is referred to as a fungus.

One of numerous thermophilic bacterial species that are a part of the Deinococcota phylum that can withstand high temperatures is Thermus aquaticus. Bacteria are classified as prokaryotes, which are unicellular life forms. Prokaryotes lack a membrane-bound nucleus and other internal components.

For more information regarding prokaryotes, visit:

brainly.com/question/1288013

#SPJ1

4 0
2 years ago
Which two scientists worked together to develop a three-dimensional model of DNA?
Oxana [17]
The last one.) watson and crick
8 0
3 years ago
Read 2 more answers
Where is kupffers cells occurs
Genrish500 [490]

They are located in the liver the wall of the sinusoids.

7 0
3 years ago
Read 2 more answers
Algae Claim
____ [38]

Answer:

The jellyfish population may have increased because of an increase in phytoplankton. This leads to an increase in   zooplankton; a decrease in walleye pollock, leading to an increase in zooplankton; or an increase in orcas, leading to a  decrease in sea turtles. Sea turtles, being the main predator for keeping the jellyfish population in check.

Also, if there are more red algae, jellyfish polyps have less place to grow. Without it, the polyps can attach itself on every surface.

Explanation:

7 0
3 years ago
Which of the following is an example of an indirect observation?
Neko [114]

Answer:

all of them okay!!!!!!! all of them !!!!!!!!!!

Explanation:

7 0
3 years ago
Other questions:
  • Simone is training for a swim race. How can she make sure she always trains at the same speed of 2 miles per hour? Check all tha
    7·1 answer
  • What is action potential
    15·1 answer
  • PLEASE HELP ME ASAP BECAUSE IT IS DIFFICULT TO UNDERSTAND. Explain it for me.
    7·1 answer
  • Metabolism is ____?
    5·2 answers
  • SCIENCE HELP 10 POINTS <br><br> ANSWER BOTH QUESTIONS PLEASE
    10·1 answer
  • And son
    13·1 answer
  • Question 1 of 10
    10·2 answers
  • GIVING BRAINLEIST!!!!!!
    13·2 answers
  • What is binary fission?
    11·2 answers
  • The right and left halves of Shawna's cerebrum can no longer communicate with each other because her _____ was destroyed. Group
    10·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!