1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Over [174]
3 years ago
5

Scientists like to use fluorescent proteins for various types of recombinant DNA procedures. You have a very small amount of the

gene for a fluorescent protein. You'd like to make a fluorescent bacterium. Which of the following represents the correct sequence of procedures that you would use?
a. transform the vector into bacteria. Amplify the gene using PCR. Insert the gene into a plasmid vector.

b. Amplify the gene using PCR. Insert the gene into a plasmid vector. Transform the vector into the bacteria.

c. Amplify the gene using PCR. Transform the vector into the bacteria. Insert the gene into a plasmid vector.

d. Insert the gene into a plasmid vector. Amplify the gene using PCR. Transform the vector into the bacteria.
Biology
1 answer:
Novosadov [1.4K]3 years ago
6 0

Answer:

The correct answer is b.Amplify the gene using PCR. Insert the gene into a plasmid vector. Transform the vector into the bacteria.

Explanation:

If I have a very small amount of gene for a fluorescent protein than the first step is to amplify the gene so that appropriate protein can be produced. PCR is the instrument that is used to amplify the protein.

So after amplification of the gene, the plasmid vector will be used in which the gene will be inserted because the plasmid vector is used to insert this gene in host cells where protein will be expressed.

The final step will be to transform bacteria with recombinant plasmid so that plasmid can make its copy and express a fluorescent protein in bulk.

You might be interested in
Flow
natka813 [3]

Answer:

Fish utilise a counter-current system in order to maximise the amount of oxygen that can extracted from water. Water has a much lower oxygen content than air and as such fish need a much more efficient system that can extract a much higher proportion of oxygen from the surrounding water.

Explanation:

6 0
3 years ago
Help me pls, What is cellulose used for?
Kisachek [45]

Answer:

Cellulose is the main substance found in plant cell walls and helps the plant to remain stiff and strong. Humans cannot digest cellulose, but it is important in the diet as a source of fibre.

Explanation:

6 0
3 years ago
Read 2 more answers
Is this correct pls help it’s timed ;( xD
aleksley [76]
Truee;)) hope it helps
5 0
3 years ago
Which pathway is generally followed by a protein produced for secretion? select one:
bazaltina [42]
<span>The correct answer is "a" - rough er, vesicle, golgi, and then vesicle. This is due to the cells shearing the rough er into microsomes, passing into a vesicle, before moving through the golgi. These newly created secretions are then treated with proteases.</span>
8 0
3 years ago
Which of these can be used to see how climate has changed over time? A. ice cores B. tree rings C. both of these
Brut [27]
I think the answer to this is both. 
5 0
3 years ago
Other questions:
  • Hector is back from his morning run and is feeling lightheaded because his energy is depleted. Which food item will provide him
    9·2 answers
  • What can be said about the phylogeny of the early hominins? a. We do not have a secure, agreed upon phylogeny for early hominins
    6·1 answer
  • What is ascites? Select one:
    6·1 answer
  • Please help will mark the brainliest
    5·1 answer
  • What most directly causes hypertension?
    9·1 answer
  • Water's ___________ makes it an excellent solvent for salts, like sodium chloride, as well as other substances required by cells
    15·2 answers
  • How can technology be used to save nature
    11·2 answers
  • The total area of land and water ecosystems an individual needs to obtain food, shelter, home heating, travel, and waste absorpt
    14·1 answer
  • Guys I need help pls
    14·1 answer
  • Help please!!!!!
    7·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!