Scientists like to use fluorescent proteins for various types of recombinant DNA procedures. You have a very small amount of the
gene for a fluorescent protein. You'd like to make a fluorescent bacterium. Which of the following represents the correct sequence of procedures that you would use? a. transform the vector into bacteria. Amplify the gene using PCR. Insert the gene into a plasmid vector.
b. Amplify the gene using PCR. Insert the gene into a plasmid vector. Transform the vector into the bacteria.
c. Amplify the gene using PCR. Transform the vector into the bacteria. Insert the gene into a plasmid vector.
d. Insert the gene into a plasmid vector. Amplify the gene using PCR. Transform the vector into the bacteria.
The correct answer is b.Amplify the gene using PCR. Insert the gene into a plasmid vector. Transform the vector into the bacteria.
Explanation:
If I have a very small amount of gene for a fluorescent protein than the first step is to amplify the gene so that appropriate protein can be produced. PCR is the instrument that is used to amplify the protein.
So after amplification of the gene, the plasmid vector will be used in which the gene will be inserted because the plasmid vector is used to insert this gene in host cells where protein will be expressed.
The final step will be to transform bacteria with recombinant plasmid so that plasmid can make its copy and express a fluorescent protein in bulk.
