Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
The third one and the last one.
Answer:
does it have water and sun through the hole?
Explanation:
if not it will die. if you specify i will edit my answer.
Answer:
Total cholesterol score is 199
199 is high cholesterol score especially as it very close to 200. The patient should adopt diets that do not include high cholesterol food
Explanation:
a. Firstly, we want to calculate the patient’s total cholesterol score.
To get this, we use a mathematical approach.
Mathematically;
the total cholesterol score = HDL + TDL + Triglyceride = 62 + 77 + 60 = 199
The patient’s total cholesterol score is 199
b. As a doctor, I will advice the patient on the status of these measurements. Using the scale of cholesterol level, a measurement of total cholesterol = 200 represents a high cholesterol level. Since 199 is very close to 200, the patient is at a risk of high cholesterol levels and thus needs a dietary change.
