The correct answer is adding ninhydrin to developing solvent.
Ninhydrinis used to detect (color) amino-acids, since amino acids are colourless compounds. So, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. It reacts with α- amino acids that results in purple coloured spots.
The biological processes that account for the difference is Meiosis and recombination.
Meiosis ensures that all organisms produced through asexual reproduction contain the correct number of chromosomes and also produces genetic variation by the process of recombination. This happens during meiosis 1 where cross over or recombination takes place, genetic material is exchanged between non sister chromosomes. Cross over creates new combinations of genes in the gametes that are not found in either parent, causing genetic diversity.
I belive its called Endosteum.