Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Parotid beacuse it include both sublingual and submandible and present near the ear
Answer:
A
Explanation:
The hypothalamus is a portion of the brain particularly concerned with homeostasis; it influences the action of the medulla oblongata, a lower part of the brain, the autonomic nervous system, and the pituitary gland.
The answer is B
Here is a small experiment you can do to test this.
<span>
Put a pot of water on the stove and bring it to a boil. Take a dry lid and cover it for a minute, and lift the lid up. What happens?
What should happen is that the water droplets run down the lid and fall back into the pot.</span>
Answer:
Pancreas secrets two hormones called insulin and glucagon which helps in maintaining the glucose level in the blood.
Explanation:
Pancreas secrets a hormone called as insulin, which helps the cell in absorbing the glucose by reducing the blood sugar and providing glucose for energy. On falling of the sugar level pancreas releases a hormone called glucagon. This glucagon informs the pancreas about the presence of low sugar in blood, after which it releases the stored glucose, and helps in raising the blood sugar level. Alpha cell of pancreas produces glucagon.
