Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
C. Trees
Explanation:
Trees can be replanted and we can grow more, even though it takes a long time this still makes them a renewable source.
Answer:
More info given below.
Explanation:
This attraction occurs as a result of static charge. You see an atom is referred to as the smallest particle of a substance; and when an atom gains or looses electrons it becomes unstable creating a difference in potential. However in this case rubbing induces friction which induces heat. This heat excites the atom to loose electrons creating a negative charge on the silk and a converse positive charge on the pencil.
Answer: i'm pretty sure its B!!!
Explanation:
...
Answer:
Hence the correct answer is Blood flow is shifted from the brain to the extremities.
Explanation:
Blood flow is shifted from the brain to the extremities - most marine mammals have an in-depth network of blood vessels towards their brain which is understood because of the retia mirabilia. These reduce the temperature in the extremities.
