Answer: C. Medulla and Cortex
The nephrons are found at the cortex-medulla junction. There are what we call as the juxtamedullary nephrons and cortical nephrons referring to their designated locations. The juxtamedullary nephrons are involved in the creation of concentrated urine of a person.
Explanation:
1. A decrease in the number of greenhouse gases that humans produce will result in lowered global temperatures. This will allow the ice sheets in the polar regions to increase increasing also albedo.
2. Using light-colored building materials on houses and pavement in urban centers will work towards increasing albedo as more sunlight is refelcted back by built environments.
3. Decreased deforestation/increase in aforestation increases the earth’s sirface albedo because vegetation reflects back more sunlight than the earth’s bare surface.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
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Answer:
Membrane proteins perform a variety of functions vital to the survival of organisms: Membrane receptor proteins relay signals between the cell's internal and external environments. Transport proteins move molecules and ions across the membrane.
Explanation:
they help transport some substances through the membrane.
