Restriction enzymes (endonucleases) are used to cut the DNA into fragements.
Explanation
Restriction enzymes are obatained from bacteria where they are utilized by bacteria for protection against viruses. Restriction enzymes are used in biotechnology research. There are several restriction enzymes and each cut the DNA at specific site known as recognition site which is usually 4-8 neucleotide long. They produce sticky ends (cutting the both DNA strand on different site) and blunt ends (cutting the both DNA strand on same site) on DNA fragments.
The fertilized cell that results is referred to as a zygote. A zygote is diploid cell, which means that it has twice the number of chromosomes as a gamete
