Question

To introduce a particular piece of DNA into an animal cell, such as that of a mouse, you would find more probable success with which of the following methods?
A) the shotgun approach
B) electroporation followed by recombination
C) introducing a plasmid into the cell
D) infecting the mouse cell with a Ti plasmid
E) transcription and translation

Answer 1

The correct answer is option B, that is, electroporation succeeded by recombination.  

A usual method of presenting exogenous nucleic acids into cells is known as electroporation. In this, an electric voltage potential is used through a biological membrane, ultimately resulting in the formation of conducting hydrophilic pores. The process of electroporation is utilized for transporting or transfecting nucleic acids generally DNA within the cells, to make them articulate genes that would modify the genetic mechanism.  

The transfected DNA can get recombined or incorporated into the host genome or can remain in its own composition. Thus, the process of electroporation straightforwardly permits more recombination to take place.