The Ames test, named for its designer, Bruce Ames, is a technique to test synthetic compounds for their disease causing properties. Three analyzer strains are utilized for this reason - Escherichia coli , Salmonella typhimurium strain An and strain B. E. coli distinguishes base pair substitution change though Salmonella typhimurium strain B identifies frameshift transformation and strain A recognizes base pair substitution change. Acridine orange intercalates itself between adjoining bases and contorts DNA helix, subsequently causes additions and erasures during replication process. Along these lines it is utilized to decide frameshift change and this is the reason it seems mutagenic in Salmonella strain B yet not strain A. Sodium nitrite deaminates cytosine to deliver uracil and uracil thus matches with adenine shaping a CG to TA progress. Along these lines it is utilized to decide base pair substitution and that is the reason it seems mutagenic in Salmonella strain A yet not recolor B.
Cellulose is plant constituent composed of a huge number of glucose units. It is the main structural component of the primary cell wall of plants. Cellulose is usually in mixture with hemicellulose, lignin and pectin. Because of its structure, cellulose is responsible for the strenght and rigidity od plant cells.