Answer: Yes.
Explanation:
Fermentation is a general term denoting anaerobic degradation of glucose or other organic nutrients in various products to obtain energy in the form of ATP. Fermentation occurs when, after glycolysis, the Krebs cycle is not performed, because the organism does not have it or because this pathway is blocked, such as during hypoxia (lack of oxygen). Fermentation is a process used by bacteria to obtain energy, does not use oxygen and occurs in the cytoplasm of cells, ie an intracellular process, and each step is catalyzed with the help of different enzymes.
Some industries use this process in the production of some products, known to all of us, such as:
1. yogurt is produced by the famous lactic fermentation, where bacteria called lactobacilli to produce lactic acid.
2. bread and beer are produced by alcoholic fermentation, where the fermentation is carried out by fungi (facultative anaerobic), which ultimately produce alcohol;
3. vinegar to that produced by acetic fermentation, which consists of a chemical reaction, where partial oxidation of ethyl alcohol occurs, obtaining acetic acid.
LolsbdgsbcgcsnnsjzkmcjMCk
Answer:
The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
Explanation:
- Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
- These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
- Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
- Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
- At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
- This can cause the protein to get denatured and hence non-functional.
- Hence, it is never advisable to preserve REs by freezing directly without glycerol.
- Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.
The nurse found a transitory
short and long term memory loss and confusion. Electroconvulsive therapy is a kind of a
procedure that done under general anesthesia,
this procedure can changes the brain chemistry by giving the patient a
small electric current that will pass through the brain intentionally making a
small fast seizure. That’s why there are some side effects is memory loss, some
memory problem improve after the treatment ends.
