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snow_tiger [21]
3 years ago
13

In blue-white screening, what do blue colonies represent?

Biology
1 answer:
Basile [38]3 years ago
3 0

Answer:

The correct option is : cells containing empty plasmid vectors

Explanation:

The blue-white screen is a technique which involves the rapid identification of the recombinant bacteria in a vector-based molecular cloning experiment. In this method, a DNA ligated vector is inserted in a host cell which is viable for transformation and grown in presence of X-gal.    

The cells that are transformed with the vectors having the recombinant DNA produce the white colonies. Whereas, the cells transformed with empty vector i.e. the non-recombinant plasmids, produce the blue colonies.

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Is a knife a biotic factors
Mama L [17]

Answer:

No

Explanation:

A knife is not a biotic factor because a biotic factor is a living organism that  shapes its enviroment.

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3 years ago
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What is sublimatoin
WINSTONCH [101]
A sublimation is a type of phase transition, or a change in a state of matter, just like melting, freezing, and evaporation.
8 0
4 years ago
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1. The complete breakdown of glucose in the presence of oxygen occurs in two stages: glycolysis and (). The first of these stage
Aliun [14]

Answer:

The complete breakdown of glucose in the presence of oxygen occurs in two stages: glycolysis and citric acid cycle. The first of these stages occur in the cytoplasm of the cell, and the second stage occurs in organelles called Mitochondria.

Explanation:

The breakdown of glucose occurs in two stages. The first stage occurs inside the cytoplasm, it is called glycolysis. when a molecule of glucose undergoes glycolysis, the following are produced:

2 ATP (Adenosine triphosphate) molecules;

2 water molecules;

2 pyruvate molecules and;

2 NADH (Nicotinamide Adenine dinucleotide) molecules

The second stage occurs in an organelle called Mitochondria, it is called citric acid cycle or tricarboxylic acid or Kreb's cycle. In this stage, the two pyruvate molecules from the glycolytic cycle are reduced to carbon(iv)oxide. The following are produced:

2 ATP molecules;

6 NADH molecules;

2 FADH2 (flavine adenine dinucleotide) and;

4 molecules of carbon(iv) oxide.

The NADH and FADH2 molecules are used in the electron transport chain generates as much as 28 ATP molecules.

4 0
3 years ago
1 What is the description of heat flow in the situation above?
Blizzard [7]

1. The answer is; B

This is because the soup has a higher temperature than the surrounding air. The heat will move from hotter object to the cooler object in search of thermal equilibrium. Heat transfer will only stop when the soup and the surrounding air have more or less the same temperatures.


2. The answer is; B

No mas is required because radiation (which are mainly electromagnetic waves) can transfer energy even through a vacuum. Other forms of energy transfer (such as convection and conduction)involve the transfer of vibrational motion from one molecule of matter to the next.


3. The answer is; D

The sun rays (which are electromagnetic waves that travel in photons) travel from the sun and through the vacuum of space before they reach the earth. When the photons hit the molecules that make up your skin, they energize them and cause them to vibrate more energetically. As they, differentially, lose this kinetic energy (when resuming a lower excitation state), this energy is expended as heat which you feel on your face.


4. The answer is; D

To start with, it is important to note that water is not a good conductor of heat. Therefore, as it is heated from below by the Bunsen burner, the water at the bottom of the glass gets warmer that than at the top of the glass. Warm water is less dense than cool water hence begin to rise while the cooler water from the top replaces the rising warm water. This process continues and forms convection currents until the water starts to boil.


5. The answer is; B

When the can is opened, it exposes the soup to the environment. This way the heat from the soup (due to thermal disequilibrium) transfers from the soup to the air. Because the soup is hotter, it loses heat energy to the surrounding as dictated by laws of thermodynamics.



4 0
3 years ago
How do the two types of fermentation differ?one produces atp and one does not. they have different end products. they have diffe
Lana71 [14]
<span>Lactic Acid Fermentation and Alcoholic Fermentation. Lactic Acid fermentation produces lactic acid, and alcoholic fermentation produces alcohol and carbon dioxide.Lactic acid fermentation has 2 ATP. Oxygen is required for the process to occur-Aerobic means. No oxygen is required for the process to occur-Anaerobic means. The ferment sugar which produces ethanol, methanol, and only a little ATP.</span>
7 0
3 years ago
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