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snow_tiger [21]
3 years ago
13

In blue-white screening, what do blue colonies represent?

Biology
1 answer:
Basile [38]3 years ago
3 0

Answer:

The correct option is : cells containing empty plasmid vectors

Explanation:

The blue-white screen is a technique which involves the rapid identification of the recombinant bacteria in a vector-based molecular cloning experiment. In this method, a DNA ligated vector is inserted in a host cell which is viable for transformation and grown in presence of X-gal.    

The cells that are transformed with the vectors having the recombinant DNA produce the white colonies. Whereas, the cells transformed with empty vector i.e. the non-recombinant plasmids, produce the blue colonies.

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The answer would be C.
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The correct answer is fever
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On a backpacking trip, Kenny hikes all day at a steady pace, covering 30 kilometers and burning 4000 Calories. At the school tra
olganol [36]

Answer:

A. If the aerobic pathway—cellular respiration—cannot meet the energy demand, then the anaerobic pathway—lactic acid fermentation—starts up, resulting in lactic acid buildup and "oxygen debt."

C. After about 90 seconds of intense exercise, the muscles become depleted of oxygen, and anaerobic respiration can no longer function to produce ATP, resulting in "oxygen debt."

Explanation:

There are two sources of carbohydrates in the human's body for energy (ATP) production. 1) Creatine phosphate and 2) Glycogen. Creatine phosphate metabolizes easily and yields ATP quickly. Whereas glycogen is stored form of carbohydrate which yields energy more slowly. Therefore, initially, our bodies use creatine phosphate and then shift to glycogen. Within 60-90 seconds, the creatinine phosphate in the body is mostly utilized and then energy is produced by the use of glycogen in aerobic pathway. During areobic pathway, oxygen supply is sufficient and per cycle, it produces 32 molecules of ATP. However, when oxygen supply is limited or absent, the body will metabolize glycogen to lactic acid via fermentation and produce only 2 molecules of ATP.

Now consider the example: Kenny hikes all day at a steady pace therefore the supply of oxygen is sufficient for aerobic cellular respiration for ATP production. In this scenario, the oxygen debt is minimal and Kenny relies on aerobic respiration pathway to obtain energy. On the other hand, Janelle runs fast (100 meters in 13.5 seconds) and her cellular respiration would be on the compense of aerobic pathway initially which will be shifted to anaerobic pathway after the supply of oxygen is reduced/minimum. Janelle will heavily rely on the anaerobic pathway because running fast needs energy which cannot be provided via aerobic pathway easily. Therefore, Janelle's body will produce lactic acid and suffer from oxygen debt.

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Answer:

when she sees the dog charging towards her her eyes sees it and sends message to the brain and then brain gives the response to run away but after running sometimes she realises that she has left the dog far so her brain responds to slow down so, after a while her heart beats also slow down.

<em><u>hope</u></em><em><u> </u></em><em><u>it helps</u></em><em><u>.</u></em><em><u>.</u></em>

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Well, the body has a endless supply of energy (even if you have an energy drink or a lot of coffee) but anyway we will always have a lot of energy until you have to go to the other world (if you know what I mean) 
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