A phage with a genome of 10000 base pairs has cot 1/2 value of 5 ,then a phage with genome of 5000 base pairs has cot 1/2 value of 2.5 .
what is genome ?
a genome is an organism's complete set of genetic instructions .each genome contains all of the information needed to build that organism and allow it to grow and develop.
haploid set of chromosomes in a cell constitutes the genome of the species .if there are two (diploid) sets of chromosomes ,there are two genomes ,one maternal and other parental .in human genome DNA is packed in 23 chromosomes .
The study of genomes and genes based on DNA sequencing is called genomics .
Human genome contains 3164.7 million nucleotide bases.
Genome contains all the genetic informations of an organism.
What is cot 1/2 ?
The product of Co ( the original concentration of denatured DNA ) and t (time in seconds ) ,givin g a useful index of DNA renaturation .Cot1/2 is the value when 50% renaturation has occurred which can be used to estimate the length of unique DNA in a sample .
A sigmoid curve which can be characterised by the Cot1/2 value ,where 1/2 of the DNA is still single stranded.
The x-axis is a log scale of the product of the initial concentration of DNA ( in moles /liter ) multiplied by length of time the reaction proceeded
(in seconds).
High Cot value indicates more number of unique sequences or less number of repetitive sequences.
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