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Freshwater biomes are large areas consisting of plants and animals grouped around water with "less than 1% salt concentration." This includes lakes, rivers, ponds, streams, and some types of wetlands.
The mutation<span> caused by the addition of a nucleotide to an already existing gene sequence is duplication.</span>
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The activity factor was modified. Ambulating indicates that the person is active. Even small activities require energy. So, people who can walk around and aren’t bedridden expend more calories than people who need to be on bedrest all day. Also, when the stress factor was changed to severe infection, the caloric requirement increased. This increase is probably because the immune system requires more energy to fight off the infection
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