It allows flexible movement that is controlled. Please mark Brainliest!!!
DNA polymerase is a type of enzyme that is responsible for forming new copies of DNA, in the form of nucleic acid molecules. Polymers are Nucleic Acid, which are large molecules made up of smaller, repeating units that are chemically connected to one another.
Answer:
2.it has gained extra electron
Answer:
Slow freeze-quick thaw is lysis protocol used widely for mammalian and bacterial cells. It works by dehydrating the sample through crystal formation.
Explanation:
Slow Freeze - Quick Thaw:
- A cell lysis procedure that involves slowly freezing cells in dry ice or ethanol or even liquid nitrogen (-196°C) and then quickly thawing them at room temperature.
- Slow freezing involves cooling below freezing point to form ice crystals in the extracellular environment. As water around the cell freezes, the presence of high concentration of solutes (salt and sugar) and cryoprotective agents induces a hypertonic environment outside the cell. This forces water outside the cell, consequently dehydrating it. Crystals also form in the cell wall, disrupting the cellular transport.
- Upon quick thawing, as the extracellular environment turns hypotonic, water rushes into the cells which swell up and burst due to osmotic shock.
