Civilizations in the Americas and sub-Saharan Africa were isolated from Eurasia mostly because of oceans and large seas such as the Mediterranean.
New England town meetings and the establishment of the Virginia house of burgesses represented the first efforts to create the American unity against the abuses of the British Empire. It was the organization of angry feelings against the lack or representatives, given that they only accepted being taxed by their own elected representatives. Those new establishments represented the weakening of the connecting between colonies and the Mother country (UK).
The Missouri compromise. Slavery not allowed passed the 36°30′ parallel.
3 because your question is still in the time of slavery, so it would be 3 I believe if not try 1.
