I am going to say a but I don’t know
Answer:
Short answer is primers are partially complementary.
Explanation:
Forward primer: 5'-AGTCTACTCGTAACCGGTTACC-3'
Reverse primer: 5'-TAAGGCATCATGGTAACCGGTT-3'
When we write reverse primer 5' to 3' we can easily see that
3'-TTGGCCAATGG---5' is complementary to the forward primers'
5'---AACCGGTTACC-3' sequence. So instead of binding to the template DNA these primers might bind each other resulting with reduction of efficiency of DNA amplification.
An aqueous environment is made up of water molecules. The word aqueous means or pertains to water or dissolved in water. In an aqueous solution, water serves as solvent. Types of aqueous solutions are acids and bases.
Answer:
The atmospheric pressure goes on decreasing as we go up above the sea-level. Therefore, the amount of oxygen also decreases at higher altitude. Due to this reason, mountaineers carry oxygen cylinders with them, while climbing high mountains
Explanation:
