There are still many Spanish speaking people there. Not only that, there are also full Hispanics there.
Answer:
While it might be found all over the world, it's rare enough that not everyone can produce them. Humans have also placed value on gold.
1. Samples of a microbial colony obtained from the natural environment are not pure culture. They are mixed cultures because the environment consists of an array of microorganisms and not one specific organism. A sample obtained from the natural environment such as water or soil may contain several species of microbes that need to be further isolated to obtain a pure culture.
2. Isolation streak plate technique involves the dilution of the sample several folds and then streaking a small amount using a sterile inoculation loop on an agar plate in a zig-zag manner. This is done to obtain a pure culture of bacteria from a mixed population by separating the microprocessor. When the sample is diluted into a small concentration and is streaked onto agar placed, widely spaced, discrete colonies of bacteria can be observed. These isolated colonies can be further cultured to obtain pure cultures by picking and re-streaking them on fresh agar plates.
3. A microbial colony is considered a pure culture because it consists of single species. A single species of bacteria cluster and form an isolated colony on an agar plate which can be further plated on fresh medium.
3-1 - If your agar plate shows discrete uncluttered colonies then you have obtained an isolated colony. The following picture might help you to understand.
3-2. If you subculture isolated microbes into a sterile nutrient broth without any contamination, it will still be considered pure culture because it is single isolated species in a sterile aseptic environment of the nutrient broth.
3.3. A pure culture can become a mixed culture due to contamination. This could be caused by airborne bacteria if your conditions are not aseptic. Contamination is possible if the work area is not clean and sterile or if the glassware is not sterile. The contamination can transform your pure culture into a mixed culture and is hence problematic. So, it is very important to maintain a sterile and aseptic environment, glassware, and instruments.
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William Lloyd Garrison, Theodore Weld, Fredrick Douglass, Sojourner Truth, Benjamin Franklin and Benjamin Rush.
Explanation:
- Abolitionists - militant opponents of 19th-century slavery in the US, also associated with European fighters against slavery.
- The abolition of slavery became a major cause of conflict between the North and the South, and it, including each state's right to decide whether to allow slavery, eventually led to the American Civil War (1861-1865).
- The first organized effort to oppose slavery occurred in 1775 when Benjamin Franklin and Dr. Benjamin Rush proved that slavery was contrary to the ideals of the Declaration of Independence and brought together the first anti-segregation group in Philadelphia.
- The founders included Thomas Payne and Alexander Hamilton.
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Http://www.un.org/un70/en/content/70ways
