Question

The human hexokinase enzyme has the same function as the bacterial hexokinase enzyme but is somewhat different in its amino acid sequence. You have obtained a mutant bacterial strain in which the gene for hexokinase and its promoter are missing. If you introduce into your mutant strain a DNA plasmid engineered to contain the coding sequence of the human hexokinase gene, driven by the normal bacterial promoter, the resulting bacteria will now produce: a) the bacterial form of hexokinase. b) the human form of hexokinase. c) a hybrid enzyme that is partly human, partly bacterial. d) both forms of the enzyme.

Answer 1

Answer:

b) the human form of hexokinase.

Explanation:

According to the given information, the amino acid sequences of bacterial and human hexokinase enzyme are different. When a human hexokinase gene sequence is introduced into a mutant bacteria that lack its own hexokinase gene sequence, the resulting bacteria will produce the human form of hexokinase. This is due to the fact that the introduced sequence was from the human hexokinase gene.

Promoters serve as binding sites for RNA polymerase to facilitate gene transcription. Therefore, the presence of a normal bacteria promoter would allow the transcription of the human hexokinase gene in the mutant bacteria.