It increased the amount of war and infighting in west Africa and depleted African resources.
The Royal Proclamation of 1763 was issued October 7, 1763, by King George III following Great Britain's acquisition of French territory in North America after the end of the French and Indian War / Seven Years' War, which forbade all settlement past a line drawn along the Appalachian Mountains.
They did it because the Ottoman Empire were suspicious of who wasn’t/ was Turkish or Muslim (something along those lines)