Prophase of mitosis do the chromosomes become distinctly visible in the microscope.
During metaphase, the nucleus dissolves and the cell's chromosomes condense and move together, aligning in the center of the dividing cell. At this stage, the chromosomes are distinguishable when viewed through a microscope. The first stage of mitosis and of meiosis I and II.
During prophase the chromosomes become visible as distinct structures, the nuclear envelope breaks down, and a spindle forms (Meiotic prophase I is more complex, and includes synapsis of homologous chromosomes and crossing over). During Prophase chromosomes become Clearly visible, nuclear envelope disappears, kinetochores and spindle fibers form.
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Answer:
<em>When focusing on a slide, ALWAYS start with either the 4X or 10X objective. Once you have the object in focus, then switch to the next higher power objective. Re-focus on the image and then switch to the next highest power.</em>
