Answer:
42,5 mL
Explanation:
We need to use the serial dilution formula beacuse we start with a stock concentrate solution and we need to prepare a new less concentrated one.
<u>DF in the dilution factor, Vi is the initial volume and Vf is the final volume.</u>
The first step is to have the same measurment unit so we need to convert 345 µg to mg.
we know that 1 µg equals 0,001 g, hence:
now the final volume is 0,345 mg protein/ mL and the inital volume is 15mg protein/mL, both of them are in the same unit so we can use the formula
Now since the question said that we already have 1.0mL of the amylase stock solution we need to subtract that 1.0mL to the 43,5 mg protein/mL
So, we need 42,5 mL of diluting buffer if we want a final concentration of 345 µg protein/mL (0.345 mg protein/mL)
Force required = F = ?
mass of the car = m = 1200 kg
initial velocity of the car = Vi = 10 m/sec
final velocity of the car = 0
time required to stop the car = t = 20 sec
Using Newton`s 2nd law of motion;
F = ma ………………………(1)
as a = Vf - Vi / t , put this value in equation (1)
F = m ( Vf - Vi /t ) ………………(2)
F = 1200 ( 0 - 10 /20) = - 600 N
here Negative sign shows that external force is acting in opposite direction of the motion to bring it to rest.
we can also calculate this force by using the relation
Rate of change of momentum = net force acting on the object …………(3)
m Vf - m Vi / t = net force acting on the object
m (Vf - Vi / t) = F
1200 ( 0 - 10 /20) = - 600 N = F
so, net force acting on the object =F = - 600 N
<span>Your body stores energy as glycogen in muscle tissue and the liver. This glycogen is stored glucose(a carbohydrate).</span>
