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VashaNatasha [74]
3 years ago
15

You can make a cell extract that is able to perform glycolysis in vitro (in a test tube) if glucose is added. Arsenate is a pote

nt inhibitor of triose phosphate dehydrogenase, the enzyme required for the 6th step in glycolysis. If both arsenate and glucose are added to the cell extract, what happens?
a. ATP levels decrease.
b. Both ATP and pyruvate levels decrease.
c. Both ATP and pyruvate levels increase.
d. ATP levels increase.
Biology
1 answer:
SIZIF [17.4K]3 years ago
7 0

Answer: B

Explanation:

If glucose and arsenate are both added to the cell extract, at first glycolysis will start.

In step one of glycolysis, glucose is phosphorylated to glucose-6-phosphate catalyzed by hexokinase which splits the ATP into ADP, and the Pi is added on to the glucose.

In step 3 of glycolysis, fructose-6-phosphate is further phosphorylated to fructose 1,6-bisphosphate. The enzyme is phosphofructokinase. This again involves hydrolysis of another ATP molecule.

A total of two ATP is used.

Step 6 in glycolysis reaction which involves generation of 2 ATP's molecules is inhibited by arsenate. Hence all other glycolytic reaction would not take place. Therefore no ATP is produced and pyruvate is not produced also.

ATP level decreases because ATP is only used up but no ATP is gained from the inhibited pathway. Also the inhibition of the step 6 enzyme cut short the pathway and pyruvate the end product of the pathway is not formed.

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In chickens, comb shape is determined by genes at two loci (R, r and P, p). A walnut comb is produced when at least one dominant
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Answer and Explanation:

<em><u>Available data</u></em>:

  • Comb shape is determined by genes at two loci (R, r and P, p).
  • The walnut comb genotype is R_P_.
  • The rose comb genotype is R_pp.
  • The pea comb genotype is rrP_.
  • The single genotype is rrpp.

a. <em>Walnut crossed with single produces 1 walnut, 1 rose, 1 pea, and 1 single offspring: </em>

Parental)             RrPp       x          rrpp

Gametes)   RP   Rp   rP   rp     rp   rp   rp   rp

Punnet Square)      RP       Rp     rP        rp

                     rp   <em>RrPp    Rrpp   rrPp   rrpp</em>

                     rp    RrPp    Rrpp   rrPp   rrpp

                     rp    RrPp    Rrpp   rrPp   rrpp

                     rp    RrPp    Rrpp   rrPp   rrpp

F1 phenotype: 25% walnut, 25% rose, 25% pea, and 25% single.

F1 genotype: 4/16 RrPp, 1/16 Rrpp, 4/16 rrPp, 4/16 rrpp.

b. <em>Rose crossed with pea produces 20 walnut offspring</em>.

Parental)              RRpp       x          rrPP

Gametes)   Rp   Rp   Rp   Rp     rP   rP   rP   rP

Punnet Square)      Rp       Rp     Rp        Rp

                     rP    RrPp    RrPp   RrPp   RrPp

                     rP    RrPp    RrPp   RrPp  RrPp

                    rP    RrPp    RrPp   RrPp   RrPp

                     rP    RrPp    RrPp   RrPp   RrPp

F1 phenotype: 100% walnut.

F1 genotype: 16/16 RrPp.

c. <em>Pea crossed with single produces 1 single offspring</em>.

This is not possible, because the pea genotype involves <u>at least</u> one dominant allele P. There are two possible crosses: <em>rrPp x rrpp</em>, which must produce half of the progeny pea and the other half single, or <em>rrPP x rrpp</em> which produce a whole pea progeny with no single offspring.  

Parental)              rrPp       x          rrpp

Gametes)   rP   rp   rP   rp     rp   rp   rp   rp

Punnet Square)     rP       rp       rP      rp

                     rp   <em>rrPp    rrpp   rrPp   rrpp</em>

                    rp    rrPp    rrpp   rrPp   rrpp

                     rp    rrPp    rrpp   rrPp   rrpp

                     rp    rrPp    rrpp   rrPp   rrpp

F1 phenotype: 50% pea, and 50% single.

F1 genotype: 8/16 rrPp, 8/16 rrpp.

d. <em>Rose crossed with pea produces 2 walnut, 1 single, and 1 pea offspring</em>.

This is not possible, because having one of the parents with a rose phenotype  involves <u>at least one R allele</u>, which means that <u>there must be rose phenotype</u> in the progeny.

Parental)             Rrpp       x          rrPp

Gametes)   Rp   Rp   rp   rp     rP   rP   rp   rp

Punnet Square)     Rp       Rp       rp      rp

                     rP  <em> RrPp </em>   RrPp  <em> rrPp</em>   rrPp

                     rP   RrPp    RrPp   rrPp   rrPp

                     rp    <em>Rrpp</em>    Rrpp   <em>rrpp </em>  rrpp

                     rp   Rrpp    Rrpp   rrpp   rrpp

F1 phenotype: 25% walnut, 25% rose, 25% pea, and 25% single.

F1 genotype: 4/16 RrPp, 1/16 Rrpp, 4/16 rrPp, 4/16 rrpp.

e. <em>Rose crossed with single produces 31 rose offspring</em>.

Parental)              RRpp       x          rrpp

Gametes)   Rp   Rp   Rp   Rp     rp   rp   rp   rp

Punnet Square)     Rp       Rp       Rp      Rp

                     rp    Rrpp    Rrpp   Rrpp   Rrpp

                     rp    Rrpp    Rrpp   Rrpp   Rrpp

                     rp    Rrpp    Rrpp   Rrpp   Rrpp

                     rp    Rrpp    Rrpp   Rrpp   Rrpp

F1 phenotype: 100% rose (31 individuals equal 100% of the progeny).

F1 genotype: 16/16 Rrpp.

f. <em>Rose crossed with single produces 10 rose and 11 single offspring.</em>

Parental)              Rrpp       x          rrpp

Gametes)   Rp   Rp   rp   rp     rP   rP   rp   rp

Punnet Square)      Rp       Rp       rp      rp

                     rp    Rrpp    Rrpp   rrpp   rrpp

                     rp    Rrpp    Rrpp   rrpp   rrpp

                     rp    Rrpp    Rrpp   rrpp   rrpp

                     rp    Rrpp    Rrpp   rrpp   rrpp

F1 phenotype: 50% rose, 50% single.

F1 genotype: 8/16 Rrpp, 8/16 rrpp.

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