When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Atherosclerosis refers to the build-up of fats and cholesterol in the walls of blood vessels (Option A).
<h3>What is atherosclerosis?</h3>
Atherosclerosis is a disease characterized by the build-up of fats and cholesterol in the walls of blood vessels.
This build-up of fats narrows the blood vessels, thereby difficulting the movement of blood in the bloodstream.
The symptoms of atherosclerosis include, among others, chest pain, shortness of breath and fatigue.
Learn more about atherosclerosis here:
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Acidophiles is the correct
C.) Electron can have a <span>charge of -1
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The large cell develop into mature gamete call ovum and it cytoplasm from the egg. The unequal distribution of the cytoplasm during oogenesis is necessary as the zygote that results in the cytoplasm from the egg. So the egg needs to have as much cytoplasm as possible
