Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
Well now not only are you killing less aqua animals. But as well as you are affecting less drinking water. But the main town supply of fish and water come from there, thus making it dangerous and hazardous after being infected.
Hope this helps
Respiratory system is refers to as the organs which are involved in breathing such as nose, throat, larynx, trachea, bronchi, and lungs. It is also known as respiratory tract.
The circulatory system is refer to as which contains the heart and the blood vessels and blood moves throughout the body in vessels. This circulatory system helps tissues to get enough oxygen and nutrients, and it helps them get rid of waste products.
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What is breathing?</h3>
When person inhale (breathe in), air enters in the lungs, and oxygen from atmosphere moves to body blood. At the same time, carbon dioxide, a waste gas, moves from blood to the lungs and is exhaled (breathed out).
Breathing process is known as gas exchange which is is essential to life.
For more information regarding breathing, visit:
brainly.com/question/15001725
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