There are no true tropic climates
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
<u>Answer:</u>
All cells have same genes, but different genes are active in different cells
<u>Explanation:</u>
Most living organisms are made up of different kinds of cells specialize in performing various functions like nerve cells perform different functions than blood cells. But all the cells are composed of same set of genetic information.
Since biochemical function is found out mainly by some specific enzymes, different sets of genes must be turned on and off in the various cell types to perform different functions. This is how cells differentiate for different functional capabilities.
Answer:
1) In mitosis, a single cell is divided into two daughter cells while in meiosis a single cell is divided into four daughter cells.
2) Mitosis occurs in body cells in which sperm and egg cells combine and make a diploid zygote while meiosis are present in sex cells in which haploid cells are produced.
3) In mitosis, the number of chromosome is double i. e. Diploid while in meiosis the number of chromosome is half i. e. haploid.
Positive selection of t cells occur in .the thymus
