B. plastic packing
A and D are biological hazards and C is a chemical hazard
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versatile cas12k-based genetic engineering toolkit (c12kget)
- Genetic modification of micro-organisms is conducive to the selection of high production producers of high-value-added chemicals, but a deficient of genetic tools limits the industrialization of most wild species
- It is very important to develop host-independent gene editing tools which can be used for genetic modification deprived strains.
- A easily found Cas12k-based genetic engineering toolkit (C12KGET) that can result into genomic integration of small parts up to 10 kb in size with up to 100% efficiency in challenging strains.
- By using C12KGET, we accomplished the first example of highly efficient genome editing in Sinorhizobium meliloti, which was successfully solved the question of that industrial strains are difficult to genetically modify, and increased vitamin B12 production by 25%.
- Cas12k can be directly used for transcriptional regulation of genes with up to 92% efficiency due to its naturally inactivated nuclease domain
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Answer:
Independent: Purposely altered
Dependent: Changes with respect to the independent being altered
Controlled: Never changes
Explanation:
An independent variable is a variable in which does not rely on other pieces/variables of an experiment to assume a value. In other words, it does not change when other variables change. However, it can be changed intentionally by the scientist.
A dependent variable is a variable in which its change in value relies on other pieces/variables of an experiment to assume a value. So, if the indeoendent variable is changed, the dependent variable will change as well. The opposite is not true though - if the dependent variable changes (for whatever reason), the independent variable hasn't necessarily been changed.
A controlled variable is a variable that stays CONSTANT throughout an experiment. It is never changed, and nothing will change it. An example of a control variable is the number of participants in a study: no more participants are being added, and no participants are being removed. The number of participants remains STATIC throughout the study.
